Question 1

What is the correct order of draw for venipuncture using evacuated tubes?

Accepted Answer

Blood cultures, light blue (sodium citrate), red/gold (SST), green (heparin), lavender (EDTA), gray (sodium fluoride/potassium oxalate).

Question 2

Which additive is found in a lavender/purple-top tube, and what is its primary use?

Accepted Answer

EDTA (ethylenediaminetetraacetic acid); it is used for complete blood count (CBC) and hematology studies by preventing clotting through calcium chelation.

Question 3

What does a gray-top tube contain, and why is it used for glucose testing?

Accepted Answer

It contains sodium fluoride (a glycolytic inhibitor) and potassium oxalate (anticoagulant); sodium fluoride prevents cells from consuming glucose, preserving accurate glucose levels.

Question 4

What is the maximum angle at which a needle should be inserted during venipuncture?

Accepted Answer

15 to 30 degrees, bevel up, in the direction of blood flow.

Question 5

What is hemoconcentration, and what causes it during venipuncture?

Accepted Answer

Hemoconcentration is an increased concentration of larger molecules in the blood; it is caused by leaving the tourniquet on for more than one minute, which increases falsely elevated results.

Lab Procedures – CMA AAMA Exam Flashcards

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