Question 1

What is the maximum time a blood specimen should be centrifuged after collection to prevent glycolysis from affecting glucose results?

Accepted Answer

Specimens should be centrifuged within 30 minutes of collection to minimize glycolysis and preserve accurate glucose levels.

Question 2

At what angle should blood tubes be transported to prevent hemolysis and ensure proper mixing of additives?

Accepted Answer

Tubes should be transported in an upright position (vertical) to minimize agitation and reduce the risk of hemolysis.

Question 3

Which specimens must be transported in a light-protected container, and why?

Accepted Answer

Bilirubin, vitamin B12, folate, and porphyrin specimens must be protected from light because exposure causes photodegradation, leading to falsely decreased results.

Question 4

What temperature range is required when transporting specimens that must be kept chilled (cold transport)?

Accepted Answer

Chilled specimens must be transported at 2–8°C, typically by placing the tube in a mixture of ice and water.

Question 5

Which analyte requires cold transport to prevent falsely elevated results if left at room temperature?

Accepted Answer

Ammonia requires cold transport; if left at room temperature, continued metabolism by red blood cells causes falsely elevated ammonia levels.

Specimen Handling for Phlebotomy Certification (NHA)

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