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Question 1
What is the correct order of draw when collecting multiple tubes during a venipuncture?
Answer: Blood cultures, then gold/red (serum), then light blue (coagulation), then green (heparin), then lavender (EDTA), then gray (glycolytic inhibitor).
Question 2
Which tube is always collected FIRST when blood cultures are ordered along with other tubes?
Answer: Blood culture bottles (aerobic first, then anaerobic) are always drawn first to minimize contamination risk.
Question 3
When NO blood cultures are ordered, which tube is drawn first in the standard order of draw?
Answer: The light blue (sodium citrate) tube is drawn first when blood cultures are not required.
Question 4
Why must the light blue (sodium citrate) tube be drawn before EDTA tubes?
Answer: EDTA contamination from a lavender tube would chelate calcium and falsely affect coagulation (PT/aPTT) results in the light blue tube.
Question 5
In the order of draw, where do serum tubes (gold SST or plain red) fall relative to the light blue tube?
Answer: Serum tubes (gold SST and plain red) are drawn AFTER the light blue tube but BEFORE additive tubes such as green, lavender, and gray.