Question 1

What is the difference between sterilization and disinfection?

Accepted Answer

Sterilization destroys ALL microorganisms including spores, while disinfection only destroys most pathogens but does not reliably kill bacterial spores.

Question 2

What is the most reliable method of sterilization used in dental offices?

Accepted Answer

Steam autoclave (moist heat sterilization) is the most reliable and commonly used method of sterilization in dental offices.

Question 3

At what temperature and time does a standard steam autoclave sterilize dental instruments?

Accepted Answer

A standard steam autoclave sterilizes at 121°C (250°F) for 15–30 minutes, or at 134°C (273°F) for 3–10 minutes depending on the cycle.

Question 4

Which biological indicator organism is used to test the effectiveness of a steam autoclave?

Accepted Answer

Geobacillus stearothermophilus (formerly Bacillus stearothermophilus) spores are used as biological indicators to test steam autoclave effectiveness.

Question 5

What is the purpose of a biological indicator (spore test) in sterilization monitoring?

Accepted Answer

A biological indicator (spore test) confirms that the sterilization process actually killed the most resistant microorganisms, verifying that the sterilizer is functioning properly.

Infection Control for DANB Dental Assistant Certification

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